Monteleone Emanuele Ricercatore tempo determinato tipo aMedicinaBIO/11

FORMAZIONE

DIPARTIMENTO DI  BILOGIA CELLULARE E DELLO SVILUPPO, UNIVERSITA’ DI PALERMO, IT / 2007-2010

Esperienza in tecniche di microbiologia

PHD IN MEDICINA MOLECOLARE – DIP. BIOTECNOLOGIE MOLECOLARI E SCIENZE DELLA SALUTE, UNIVERSITA’ DI TORINO, IT / 2017

Tesi: “Discovery and characterization of long non-coding RNAs controlling pluripotency and self-renewal in mouse embryonic stem cells”.

LAUREA SPECIALISTICA IN BIOLOGIA CELLULARE E MOLECOLARE – DIP. DI BIOLOGIA ANIMALE E UMANA, UNIVERSITA’ DI TORINO, IT / 2012

Tesi: “Estrogen receptor alpha maintains basal activity of transcriptional enhancers in a ligand-independent manner in collaboration with other transcription factors”.

LAUREA TRIENNALE IN SCIENZE BILOGICHE – DIP. BIOLOGIA CELLULARE E DELLO SVILUPPO, UNIVERSITA’ DI PALERMO, IT / 2010

Tesi: “Expression of RBL2 in lung and breast tumoral cells”.o

ATTIVITA' SVOLTA

Bioinformatica

• Analisi funzionale: Analisi di arrichimento, Analisi basata sui gene-set.
• Analisi Microarray: espressione genica , genotipizzazione
• Analisi di Sequenziamento: esoma, varianti alleliche, RNA-seq, Meth-seq, DNA-Seq
  ATAC-Seq, Mnase-Seq
• Biostatistica: normalizzazione dati microarray, regressione, test multipli e correzione
• Predittori e clustering  di espressione genica: Classificazione e alberi di regressione, Random Forest, Cross validation, K-means, K-medoids, Clustering gerarchico
• Biologia de sistemi e analisi delle reti biologiche
• Databases e repositories: Ensembl, UCSC, MsigDB, EMBL, GeneBank, Uniprot
• Librerie: Ensembl API, SAMtools, Bioconductor, BioPerl, Biopython.\

Bilogia Cellulare e Molecolare

• Colture microbilogiche e di cellule umane e di topo, trasfezioni transienti (plasmidi, siRNA, shRNA)
• Estrazione di DNA e RNA , PCR, Real Time PCR, ChIP, Clonaggi, IF, RNA-FISH, Northern Blot, REMSA
• Estrazione di estratti totali proteici e frazionamento, Western Blot, IP
• Manipolazione dei Topi

 

ATTIVITA' DI RICERCA

MOLECULAR BIOTECHNOLOGY CENTER, UNIVERSITA’ DI TORINO, IT / 2017- 2019

Esperienza di Ricerca in Bioinformatica e Biologia dei Tumori

CENTER FOR LIFE SCIENCE, HARVARD MEDICAL SCHOOL, BOSTON, USA / 2016

Esperienza di Ricerca in Bioinformatica e Biologia dei Tumori

MOLECULAR BIOTECHNOLOGY CENTER, UNIVERSITA’ DI TORINO, IT / 2013-2015

Esperienza di Ricerca in Bioinformatica e Biologia molecolare dello sviluppo

PREMI E RICONOSCIMENTI

2019: Premio Francesca Martini (SIBBM 2019)

2016: EMBO short-term fellowship

 

1   Brambilla, F. et al. Nucleosomes effectively shield DNA from radiation damage in living cells

Nucleic Acids Research 48 (16), 8993-900 (2020).

2.     Nachmani, D. et al. Germline NPM1 mutations lead to altered rRNA 2′-O-methylation and cause dyskeratosis congenita. Nat. Genet. 1–12 (2019).

3.     Kunkl, M. et al. CD28 individual signaling up-regulates human IL-17A expression by promoting the recruitment of RelA/NF-κB and STAT3 transcription factors on the proximal promoter. Front. Immunol. 10, (2019).

4.     Monteleone, E. & Poli, V. Where Sin3a Meets STAT3: Balancing STAT3-Mediated Transcriptional Activation and Repression. Cancer Res. 79, 3031–3033 (2019).

5.     Mugoni, V. et al. Vulnerabilities in mIDH2 AML confer sensitivity to APL-like targeted combination therapy. Cell Res. 29, 446 (2019).

6.     Lee, Y.-R. et al. Reactivation of PTEN tumor suppressor for cancer treatment through inhibition of a MYC-WWP1 inhibitory pathway. Science (80-. ). 364, eaau0159 (2019).

7.     Savino, A. et al. Network analysis allows to unravel breast cancer molecular features and to identify novel targets. bioRxiv 570051 (2019).

8.     Monteleone, E. et al. SP1 and STAT3 Functionally Synergize to Induce the RhoU Small GTPase and a Subclass of Non-canonical WNT Responsive Genes Correlating with Poor Prognosis in Breast Cancer. Cancers (Basel). 11, 101 (2019).

9.     Bester, A. C. et al. An integrated genome-wide CRISPRa approach to functionalize lncRNAs in drug resistance. Cell 173, 649–664 (2018).

10.    Circosta, P. et al. Tailoring CD19xCD3-DART exposure enhances T-cells to eradication of B-cell neoplasms. Oncoimmunology 7, e1341032 (2018).

11.    Matsumoto, A. et al. mTORC1 and muscle regeneration are regulated by the LINC00961-encoded SPAR polypeptide. Nature 541, 228 (2017).

12.    Camporeale, A. et al. STAT3 activities and energy metabolism: dangerous liaisons. Cancers (Basel). 6, 1579–1596 (2014).