Grohovaz Fabio Professore ordinarioMedicinaBIO/09

Professore Ordinario di Fisiologia – Facoltà di Medicina e Chirurgia

Esperienza professionale

1978

Laurea in Chimica e Tecnologia Farmaceutiche presso l’Università degli Studi di Milano

1980

Honorary Research Assistant, Biophysics Dept, University College, London, UK.

1981

Honorary Research Fellow, Biophysics Dept, University College, London, UK.

1983-1984

Professore a contratto, Facoltà di Farmacia, Università di Trieste.

1984-2002

Ricercatore, Consiglio Nazionale delle Ricerche

Dal 1992

Responsabile Unità di Neurofisiologia Cellulare, DIBIT, Ist. Scient. S. Raffaele, Milano.

1997

Membro del gruppo insignito con l’Armenise-Harvard Foundation Neuroscience Award Italy.

2001-presente

Direttore di Alembic (Advanced Light and Electron Microscopy Bio-Imaging Center), Ist. Scient. S, Raffaele, Milano.

2002-presente

Professore Ordinario di Fisiologia, Facoltà di Medicina, Università Vita-Salute S. Raffaele, Milano.

a.a. 2002-08

Presidente del CdL in Biotecnologie Mediche e Farmaceutiche, Università Vita-Salute S. Raffaele, Milano.

a.a. 2003-08

Presidente del CdLS in Biotecnologie Mediche Molecolari e Cellulari, Università Vita-Salute S. Raffaele, Milano.

a.a. 2005-08

Responsabile del Dottorato in Biotecnologie delle Neuroscienze, Università Vita-Salute San Raffaele e Istituto Italiano di Tecnologie.

a.a. 2010-11 – presente

Direttore della Scuola di Dottorato Internazionale in Medicina Molecolare, Università Vita-Salute San Raffaele, Milano.

Aree di ricerca

. Attività sinaptica

. Segnali intracellulari

. Malattie neurodegenerative

Pubblicazioni indicizzate dal 1999

(* shared contribution, ^ corresponding author)

 

Pelizzoni I, Zacchetti D, Smith CP, Grohovaz F ^and Codazzi F^ (2012) Expression of DMT1 in primary hippocampal neurons: reconsidering its role in non-transferrin-bound iron influx. J. Neurochem. 120: 269-278.

 

Pelizzoni I, Macco R, Morini M, Zacchetti D, Grohovaz F^, and Codazzi F^ (2011) Iron handling in hippocampal neurons: activity-dependent iron entry and mitochondria-mediated neurotoxicity. Aging Cell. 10, 172–183.

 

Bettegazzi B*, Mihailovich M*, Di Cesare A, Consonni A, Macco R, Pelizzoni I, Codazzi F, Grohovaz F^, and Zacchetti D^ (2011) β-secretase Activity in Rat Astrocytes: Translational Block of BACE1 and Modulation of BACE2 Expression. Eur J Neurosci. 33, 236–243.

 

Consonni A*, Morara S*, Codazzi F, Grohovaz F^, and Zacchetti D^ (2011) Inhibition of Lipopolysaccharide-induced Microglia Activation by Calcitonin Gene Related Peptide and Adrenomedullin. Mol Cell Neurosci. 48, 151-160.

 

D’Antoni S, Zambusi L, Codazzi F, Zacchetti D, Grohovaz F, Provini L, Catania MV, Morara S^ (2010) Calcitonin Gene-Related Peptide (CGRP) stimulates Purkinje cell dendrite growth in culture. Neurochem Res. 2010 35:2135-43.

 

Morara S, Wang LP, Filippov V, Dickerson IM, Grohovaz F, Provini L, Kettenmann H. ^ (2008) Calcitonin gene-related peptide (CGRP) triggers Ca2+ responses in cultured astrocytes and in Bergmann glial cells from cerebellar slices. Eur J Neurosci. 28: 2213-2220.

 

Pelizzoni I, Macco R, Zacchetti D, Grohovaz F^, Codazzi F^ (2008) Iron and calcium in the central nervous system: a close relationship in health and sickness. Biochem Soc Trans. 36: 1309-1312.

 

Chiulli N, Codazzi F, Di Cesare A, Gravaghi C, Zacchetti^ D, Grohovaz^ F (2007) Sphingosylphosphocholine effects on cultured astrocytes reveal mechanisms potentially involved in neurotoxicity in Niemann-Pick type A disease. Eur. J. Neurosci. 26:875–881

 

Mihailovich M, Thermann R, Grohovaz F, Hentze MW, Zacchetti D (2007) Complex translational regulation of BACE1 involves upstream uAUGs and stimulatory elements within the 5′ untranslated region. Nucl. Acids Res. 35:2975-2985.

 

Zacchetti D, Chieregatti E, Bettegazzi B, Mihailovich M, Sousa VL, Grohovaz F, Meldolesi J ^ (2007) BACE1 expression and activity: relevance in Alzheimer’s disease. Neurodegenerative Dis. 4:117-126

 

Di Cesare A, Del Piccolo P, Zacchetti D^, and Grohovaz F^ (2006) EP2 receptor stimulation promotes calcium responses in astrocytes via activation of the adenylyl cyclase pathway. Cell. Mol. Life Sci. 63: 2546-2553

 

Codazzi F, Di Cesare A, Chiulli N, Albanese A, Meyer T, Zacchetti D, and Grohovaz F^ (2006) Synergistic control of Protein Kinase Cγ activity by ionotropic and metabotropic glutamate receptor inputs in hippocampal neurons. J Neurosci, 26: 3404-3411

 

Negri L, Lattanzi R, Giannini E, Colucci MA, Mignogna G, Barra D, Grohovaz F,Codazzi F, Kaiser A, Kreil G, Melchiorri P. (2005) Biological activities of Bv8 analogues.Br J Pharmacol. 146:625-32.

 

Micheletti M., Brioschi A., Fesce R., and Grohovaz F. ^ (2005) A novel pattern of fast calcium oscillations points to calcium and electrical activity cross-talk in rat chromaffin cells. Cell. Mol. Life Scie. 62: 95-104

 

De Pietri Tonelli* D, Mihailovich M*, Di Cesare A, Codazzi F, Grohovaz F and Zacchetti D (2004) Translational regulation of BACE-1 expression in neuronal and non-neuronal cells. Nucl. Acids Res. 32: 1808-1817

 

De Pietri Tonelli D, Mihailovich M, Schnurbus R, Pesole G, Grohovaz F, and Zacchetti D. (2003) Translational control of Scamper expression via a cell-specific internal ribosome entry site.Nucl. Acids Res. 31: 2508-2513

 

Schnurbus R., De Pietri Tonelli D., Grohovaz F., and Zacchetti D. (2002) Re-evaluation of primary structure, topology, and localization of Scamper, a putative intracellular Ca2+ channel activated by spingosylphosphocholine. Biochem. J. 362: 183-189

 

Bertuzzi F., Grohovaz F., Maffi P., Caumo A., Aldrighetti L., Nano R., Hengster P., Calori G., Di Carlo V., Bonifacio E., and Secchi A. (2002) Succesful transplantation of human islets in recipients bearing a kidney graft. Diabetologia 45: 77-84

 

Pezzati R., Meldolesi J., and Grohovaz F. ^ (2001) Ultra rapid calcium events in electrically stimulated frog nerve terminals. Biochem. Biophys. Res. Comm. 285:724-727

 

Meldolesi J. and Grohovaz F. (2001) Total calcium ultrastructure: advances in excitable cells. Cell Calcium. 30: 1-8

 

Lorenzon P., Grohovaz F., and Ruzzier F. (2000) Voltage- and ligand-gated ryanodine receptors are functionally separated in developing C2C12 mouse myotubes. J. Physiol. 525.2: 499-507

 

Bertuzzi F., Davalli A.M., Nano R., Socci C., Codazzi F., Fesce R., Di Carlo V., Pozza G., and Grohovaz F. ^ (1999) Mechanisms of Coordination of Ca2+ Signals in Pancreatic Islet Cells. Diabetes 48: 1971-1978

 

Tonini R., Grohovaz F., Laporta C.A.M., and Mazzanti M. (1999) Gating mechanism of the nuclear pore complex channel in isolated neonatal and adult mouse liver nuclei.Faseb J. 13: 1395-1403

 

Kasai H., Kishimoto T., Liu T.T., Miyashita Y., Podini P., Grohovaz F., and Meldolesi J. (1999) Multiple and diverse forms of regulated exocytosis in wild-type and defective PC12 cells. Proc. Natl. Acad. Sci. USA. 96: 945-9

 

Pezzati R. and Grohovaz F. ^ (1999) The frog neuromuscular junction revisited after quick-freezing-freeze-drying: ultrastructure, immunogold labelling and high resolution calcium mapping. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 354: 373-378

Full Professor of Physiology

 

Positions:

1978

“Laurea” in Pharmaceutical Chemistry and Technology, University of Milan

 

1980

Honorary Research Assistant, Biophysics Department, University College, London, UK

 

1981

Honorary Research Fellow, Biophysics Department, University College, London, UK

 

1983-1984

Lecturer, Faculty of Pharmacy, University of Trieste, Italy

 

1984-2002

Senior Research Fellow, National Council of Research (CNR)

 

1992-today

Head of the Cellular Neurophysiology Unit, DIBIT, San Raffaele Scient. Inst., Milan, Italy

 

1997

Member of the group awarded with the Armenise-Harvard Foundation Neuroscience Award Italy

 

2000-today

Director of Alembic (Advanced Light and Electron Microscopy Bio-Imaging Center), DIBIT, San Raffaele Scient. Inst.,

Milan, Italy

 

2002-today

Full Professor of Physiology School of Medicine, University Vita-Salute S. Raffaele, Milan, Italy

 

2003-today

Chairman of the Degree (Laurea) in Medical and Pharmaceutical Biotechnology, School of Medicine, University, Vita-Salute S. Raffaele, Milan, Italy

 

2004-today

Chairman of the Master of Science (Laurea Specialistica) in Molecular and Cellular Medical Biotechnology, School of Medicine, University Vita-Salute S. Raffaele, Milan, Italy.

 

2005-2008

Chairman of the PhD in Neuroscience Biotechnology, University Vita-Salute S. Raffaele and Italian Institute of Technology, Italy.

 

2010-today

Director of the International PhD School in Molecular Medicine, San Raffaele University, Milan, Italy.

 

Research interests

. Synaptic activity

. Intracellular signaling

. Neurodegenerative diseases

(* shared contribution, ^ corresponding author)

 

Pelizzoni I, Zacchetti D, Smith CP, Grohovaz F ^and Codazzi F^ (2012) Expression of DMT1 in primary hippocampal neurons: reconsidering its role in non-transferrin-bound iron influx. J. Neurochem. 120: 269-278.

 

Pelizzoni I, Macco R, Morini M, Zacchetti D, Grohovaz F^, and Codazzi F^ (2011) Iron handling in hippocampal neurons: activity-dependent iron entry and mitochondria-mediated neurotoxicity. Aging Cell. 10, 172–183.

 

Bettegazzi B*, Mihailovich M*, Di Cesare A, Consonni A, Macco R, Pelizzoni I, Codazzi F, Grohovaz F^, and Zacchetti D^ (2011) β-secretase Activity in Rat Astrocytes: Translational Block of BACE1 and Modulation of BACE2 Expression. Eur J Neurosci. 33, 236–243.

 

Consonni A*, Morara S*, Codazzi F, Grohovaz F^, and Zacchetti D^ (2011) Inhibition of Lipopolysaccharide-induced Microglia Activation by Calcitonin Gene Related Peptide and Adrenomedullin. Mol Cell Neurosci. 48, 151-160.

 

D’Antoni S, Zambusi L, Codazzi F, Zacchetti D, Grohovaz F, Provini L, Catania MV, Morara S^ (2010) Calcitonin Gene-Related Peptide (CGRP) stimulates Purkinje cell dendrite growth in culture. Neurochem Res. 2010 35:2135-43.

 

Morara S, Wang LP, Filippov V, Dickerson IM, Grohovaz F, Provini L, Kettenmann H. ^ (2008) Calcitonin gene-related peptide (CGRP) triggers Ca2+ responses in cultured astrocytes and in Bergmann glial cells from cerebellar slices. Eur J Neurosci. 28: 2213-2220.

 

Pelizzoni I, Macco R, Zacchetti D, Grohovaz F^, Codazzi F^ (2008) Iron and calcium in the central nervous system: a close relationship in health and sickness. Biochem Soc Trans. 36: 1309-1312.

 

Chiulli N, Codazzi F, Di Cesare A, Gravaghi C, Zacchetti^ D, Grohovaz^ F (2007) Sphingosylphosphocholine effects on cultured astrocytes reveal mechanisms potentially involved in neurotoxicity in Niemann-Pick type A disease. Eur. J. Neurosci. 26:875–881

 

Mihailovich M, Thermann R, Grohovaz F, Hentze MW, Zacchetti D (2007) Complex translational regulation of BACE1 involves upstream uAUGs and stimulatory elements within the 5′ untranslated region. Nucl. Acids Res. 35:2975-2985.

 

Zacchetti D, Chieregatti E, Bettegazzi B, Mihailovich M, Sousa VL, Grohovaz F, Meldolesi J ^ (2007) BACE1 expression and activity: relevance in Alzheimer’s disease. Neurodegenerative Dis. 4:117-126

 

Di Cesare A, Del Piccolo P, Zacchetti D^, and Grohovaz F^ (2006) EP2 receptor stimulation promotes calcium responses in astrocytes via activation of the adenylyl cyclase pathway. Cell. Mol. Life Sci. 63: 2546-2553

 

Codazzi F, Di Cesare A, Chiulli N, Albanese A, Meyer T, Zacchetti D, and Grohovaz F^ (2006) Synergistic control of Protein Kinase Cγ activity by ionotropic and metabotropic glutamate receptor inputs in hippocampal neurons. J Neurosci, 26: 3404-3411

 

Negri L, Lattanzi R, Giannini E, Colucci MA, Mignogna G, Barra D, Grohovaz F,Codazzi F, Kaiser A, Kreil G, Melchiorri P. (2005) Biological activities of Bv8 analogues.Br J Pharmacol. 146:625-32.

 

Micheletti M., Brioschi A., Fesce R., and Grohovaz F. ^ (2005) A novel pattern of fast calcium oscillations points to calcium and electrical activity cross-talk in rat chromaffin cells. Cell. Mol. Life Scie. 62: 95-104

 

De Pietri Tonelli* D, Mihailovich M*, Di Cesare A, Codazzi F, Grohovaz F and Zacchetti D (2004) Translational regulation of BACE-1 expression in neuronal and non-neuronal cells. Nucl. Acids Res. 32: 1808-1817

 

De Pietri Tonelli D, Mihailovich M, Schnurbus R, Pesole G, Grohovaz F, and Zacchetti D. (2003) Translational control of Scamper expression via a cell-specific internal ribosome entry site.Nucl. Acids Res. 31: 2508-2513

 

Schnurbus R., De Pietri Tonelli D., Grohovaz F., and Zacchetti D. (2002) Re-evaluation of primary structure, topology, and localization of Scamper, a putative intracellular Ca2+ channel activated by spingosylphosphocholine. Biochem. J. 362: 183-189

 

Bertuzzi F., Grohovaz F., Maffi P., Caumo A., Aldrighetti L., Nano R., Hengster P., Calori G., Di Carlo V., Bonifacio E., and Secchi A. (2002) Succesful transplantation of human islets in recipients bearing a kidney graft. Diabetologia 45: 77-84

 

Pezzati R., Meldolesi J., and Grohovaz F. ^ (2001) Ultra rapid calcium events in electrically stimulated frog nerve terminals. Biochem. Biophys. Res. Comm. 285:724-727

 

Meldolesi J. and Grohovaz F. (2001) Total calcium ultrastructure: advances in excitable cells. Cell Calcium. 30: 1-8

 

Lorenzon P., Grohovaz F., and Ruzzier F. (2000) Voltage- and ligand-gated ryanodine receptors are functionally separated in developing C2C12 mouse myotubes. J. Physiol. 525.2: 499-507

 

Bertuzzi F., Davalli A.M., Nano R., Socci C., Codazzi F., Fesce R., Di Carlo V., Pozza G., and Grohovaz F. ^ (1999) Mechanisms of Coordination of Ca2+ Signals in Pancreatic Islet Cells. Diabetes 48: 1971-1978

 

Tonini R., Grohovaz F., Laporta C.A.M., and Mazzanti M. (1999) Gating mechanism of the nuclear pore complex channel in isolated neonatal and adult mouse liver nuclei.Faseb J. 13: 1395-1403

 

Kasai H., Kishimoto T., Liu T.T., Miyashita Y., Podini P., Grohovaz F., and Meldolesi J. (1999) Multiple and diverse forms of regulated exocytosis in wild-type and defective PC12 cells. Proc. Natl. Acad. Sci. USA. 96: 945-9

 

Pezzati R. and Grohovaz F. ^ (1999) The frog neuromuscular junction revisited after quick-freezing-freeze-drying: ultrastructure, immunogold labelling and high resolution calcium mapping. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 354: 373-378