Monteleone Emanuele Ricercatore tempo determinato tipo aMedicineBIO/11

EDUCATION

PHD IN MOLECULAR MEDICINE – DEPT. MOLECULAR BIOTECHNOLOGY AND HEALTH SCIENCES, UNIVERSITY OF TURIN, IT / 2017

Training thesis: “Discovery and characterization of long non-coding RNAs controlling pluripotency and self-renewal in mouse embryonic stem cells”.

MASTER’S DEGREE IN MOLECULAR AND CELLULAR BIOLOGY – DEPT. OF ANIMAL AND HUMAN BIOLOGY, UNIVERSITY OF TURIN, IT / 2012

Training thesis: “Estrogen receptor alpha maintains basal activity of transcriptional enhancers in a ligand-independent manner in collaboration with other transcription factors”.

BACHELOR DEGREE IN BIOLOGICAL SCIENCES – DEPT. OF CELLULAR AND DEVELOPMENTAL BIOLOGY, UNIVERSITY OF PALERMO, IT / 2010

Training thesis: “Expression of RBL2 in lung and breast tumoral cells”.

RESEARCH

MOLECULAR BIOTECHNOLOGY CENTER, UNIVERSITY OF TURIN, IT / 2017- 2019

Research experience in Bioinformatics and Cancer Biology

CENTER FOR LIFE SCIENCE, HARVARD MEDICAL SCHOOL, BOSTON, USA / 2016

Research experience in Bioinformatics and Cancer Biology

MOLECULAR BIOTECHNOLOGY CENTER, UNIVERSITY OF TURIN, IT / 2013-2015

Research experience in Bioinformatics and Developmental Molecular Biology

SKILLS

Bioinformatics Skills

• Functional analysis: single enrichment analysis, gene-set based analysis
• Microarray data analysis: expression, genotyping, exon
• NGS data analysis: exome, variant calling, RNA-seq, Meth-seq, DNA-Seq,

ATAC-Seq, MNase-Seq

• Biostatistics: microarray normalization, regression, multiple test correction
• Predictors and clustering analysis of expression data: Classification and regression Tree, Random Forest, Cross validation, K-means, K-medoids, Hierachical Clustering
• System biology and biological networks analysis
• Databases and repositories: Ensembl, UCSC, MsigDB, EMBL, GeneBank, Uniprot
• Libraries: Ensembl API, SAMtools, Bioconductor, BioPerl, Biopython

Molecular and Cellular Biology

• Microbiological, Human and Mouse cell cultures, transient and stable transfections of human and mouse cells in culture (plasmids, siRNA, shRNA)
• DNA and RNA extraction, PCR, Real Time PCR, ChIP, Cloning, IF, RNA-FISH, Northern Blot, REMSA
• Whole protein extraction and fractioning, Western Blot, IP
• Animal handling (Mice)

AWARDS

2019: The Francesca Martini Prize in SIBBM 2019

2016: EMBO short-term fellowship for the US.

 

1   Brambilla, F. et al. Nucleosomes effectively shield DNA from radiation damage in living cells

Nucleic Acids Research 48 (16), 8993-900 (2020).

2.     Nachmani, D. et al. Germline NPM1 mutations lead to altered rRNA 2′-O-methylation and cause dyskeratosis congenita. Nat. Genet. 1–12 (2019).

3.     Kunkl, M. et al. CD28 individual signaling up-regulates human IL-17A expression by promoting the recruitment of RelA/NF-κB and STAT3 transcription factors on the proximal promoter. Front. Immunol. 10, (2019).

4.     Monteleone, E. & Poli, V. Where Sin3a Meets STAT3: Balancing STAT3-Mediated Transcriptional Activation and Repression. Cancer Res. 79, 3031–3033 (2019).

5.     Mugoni, V. et al. Vulnerabilities in mIDH2 AML confer sensitivity to APL-like targeted combination therapy. Cell Res. 29, 446 (2019).

6.     Lee, Y.-R. et al. Reactivation of PTEN tumor suppressor for cancer treatment through inhibition of a MYC-WWP1 inhibitory pathway. Science (80-. ). 364, eaau0159 (2019).

7.     Savino, A. et al. Network analysis allows to unravel breast cancer molecular features and to identify novel targets. bioRxiv 570051 (2019).

8.     Monteleone, E. et al. SP1 and STAT3 Functionally Synergize to Induce the RhoU Small GTPase and a Subclass of Non-canonical WNT Responsive Genes Correlating with Poor Prognosis in Breast Cancer. Cancers (Basel). 11, 101 (2019).

9.     Bester, A. C. et al. An integrated genome-wide CRISPRa approach to functionalize lncRNAs in drug resistance. Cell 173, 649–664 (2018).

10.    Circosta, P. et al. Tailoring CD19xCD3-DART exposure enhances T-cells to eradication of B-cell neoplasms. Oncoimmunology 7, e1341032 (2018).

11.    Matsumoto, A. et al. mTORC1 and muscle regeneration are regulated by the LINC00961-encoded SPAR polypeptide. Nature 541, 228 (2017).

12.    Camporeale, A. et al. STAT3 activities and energy metabolism: dangerous liaisons. Cancers (Basel). 6, 1579–1596 (2014).