Zambrano Silva Samuel Professore AssociatoMedicine02/D1





2003: B. Sc. Physics, Universidad Complutense, Madrid (Spain)

2006: MAS in Computer Science and Mathematical Modeling, Universidad Rey Juan Carlos, Madrid (Spain).

2007: Ph. D. Physics, Universidad Rey Juan Carlos, Madrid (Spain).


2003-2011: Universidad Rey Juan Carlos, Madrid (Spain).

Nonlinear Dynamics, Chaos and Complex Systems Group led by Prof. Miguel A. F. Sanjuán.

  • 2003-2007 Ph.D. student.
  • 2007-2009 Profesor ayudante doctor (Assistant Professor).
  • 2009-2011 Profesor contratado doctor (step next to Assistant Professor).
  • 2011-March 2017. Leave of absence.

2011-present: San Raffaele University and Scientific Institute (SRSI).

In Vivo Chromatin and Transcription Unit, led by Dr. Alessandra Agresti, Chromatin Dynamics Unit led by Prof. Marco E. Bianchi.

  • March 2011 – March 2012 Postdoctoral fellow (Selected in the first SRSI International Postdoctoral Programme).
  • March 2012 – March 2014 Marie Curie IEF Research Fellow.
  • March 2014 – March 2017 Postdoctoral fellow.
  • April 2017 – October 2017. Research fellow at San Raffaele University.
  • October 2017 – September 2020. Assistant professor at San Raffaele University.
  • Since October 2020: Associate professor of physics at San Raffaele University.


I started my scientific career in the fields of nonlinear dynamics and chaos, with a focus in the control of chaotic dynamics and in analysis of time series. Since I arrived to San Raffaele, I have been focusing on applications in quantitative and systems biology, in particular to understand the dynamics of NF-kB in single cells and its role in the control of transcription.


- Supervisor (PI) of a 1-year fellowship funded by San Raffaele University. Project title: “Characterization of nuclear dynamics and physical properties in myogenesis by live-cell imaging”. From January 2021.

- OSR Seed Grant. “Spatiotemporal dynamics of inflammation: role of NF-kappaB dynamics at a tissue level”. One year grant starting from March 2018 to March 2020 (PI).

– Marie Curie Intra-European Fellowship (IEF) for a postdoctoral project at San Raffaele University (Milan, Italy) “Role of Nonlinear Dynamics of NF-kB in Inflammation” (Grant Agreement 298447) (2012)

– Participation as a senior postdoc in the project funded within the 2016 Call for proposals by AIRC (Italian Association for Cancer Research). Title of the project: “NF-kB in motion: role of NF-kB dynamics in Multiple Myeloma and stromal cells interactions”. PI: Alessandra Agresti. From January 2017.

– Participation in research projects: two Spanish National research projects (BFM2003-03081 and FIS2006-08525) one European project (HI03-207) and two research projects co-funded by the Madrid Region and Universidad Rey Juan Carlos (URJC-CM-2006-CET-0643 and URJC-CM-2007-CET-1601).


– Member of the Italian Society of Molecular Biology and Biophysics since 2016, of the Italian Society of Cell and Developmental Biology and of the Italian Society of Chaos and Complexity since 2019.



Coordinator and lecturer in different physics courses for engineering and environmental sciences in the university. I also participated as lecturer in different courses in the university’s graduate program on nonlinear dynamics and complex systems.

2011-2017: SAN RAFFAELE UNIVERSITY (ITALY) 2013/2014 – 2017/2018: Lecturer, Gene Expression in 4D. Degree in Biotechnology (Italian).

2016/2017 – today: Coordinator: Physics. Degree in Biotechnology (Italian).

2014/2015 – 2017/2018 Lecturer and from 2018/2019 coordinator of Medical Physics. International MD program (English).

2013/2014 – today: Tutor, Cell and Molecular Biology. MD program (Italian and English).

2013/2014 – 2017/2018: Lecturer, Gene Expression in 4D. Degree in Biotechnology (Italian).

2015/2016 – today: Lecturer, Genetics and Developmental Biology. MD program (Italian).


Pubblicazioni selezionate/ Selected publications:

Samuel Zambrano, A. Loffreda, E. Carelli, G. Stefanelli, F. Colombo, E. Bertrand, A. Agresti, M. E. Bianchi N. Molina, D. Mazza. “First Responders Shape a Prompt and Sharp NF-κB-Mediated Transcriptional Response to TNF-α”. iScience 23(9), 101529 (2020).

G. Careccia, F. Colombo, M. Tirone, A. Agresti, M. E. Bianchi, Samuel Zambrano, E. Vénéreau. “Exploiting Live Imaging to Track Nuclei During Myoblast Differentiation and Fusion”. J. Vis. Exp. (146), e58888, (2019).

F. Colombo, Samuel Zambrano*, A. Agresti. “NF-kB, the importance of being dynamic: role and insights in cancer”. Biomedicines, 6 (2), 45 (2018).

E. Ramos-Marquès, Samuel Zambrano, A. Tiérrez, M. E. Bianchi, A. Agresti and F. García-del Portillo. “Single-cell analyses reveal an attenuated NF-kB response in the Salmonella-infected fibroblast”. Virulence 8:6, 719-740 (2017).

Samuel Zambrano, I. De Toma, A. Piffer, M. E. Bianchi and A. Agresti. “NF-kB oscillations translate into functionally related patterns of gene expression”. eLife 5:e09100 (2016).

R. Meucci, S. Euzzor, E. Pugliese, Samuel Zambrano, M.R. Gallas and J.A.C. Gallas. “Optimal Phase-Control Strategy for Damped-Driven Duffing Oscillators”. Phys. Rev. Lett. 116, 044101 (2016).

Samuel Zambrano, Marco E. Bianchi, Alessandra Agresti and Nacho Molina. “Interplay between stochasticity and negative feedback leads to pulsed dynamics and distinct gene activity patterns”. Phys. Rev. E 92, 02271 (2015).

Samuel Zambrano, M. E. Bianchi, A. Agresti. “A simple model of NF-kB dynamics reproduces experimental observations” Journal of Theoretical Biology, 347(21) 44-53 (2014).

Samuel Zambrano, M. E. Bianchi, A. Agresti. “High-Throughput Analysis of NF-kB Dynamics in Single Cells Reveals Basal Nuclear Localization of NF-kB and Spontaneous Activation of Oscillations”. PLoS ONE 9(3): e90104(2014).

J. Sabuco, Samuel Zambrano, M. A. F. Sanjuán, and James A. Yorke. “Finding safety in partially controllable chaotic systems”. Commun. Nonlinear Sci. Numer. Simulat., 17:4274-4280 (2012).

Samuel Zambrano, I. P. Mariño, J. M. Seoane, M. A. F. Sanjuán, S. Euzzor, A. Geltrude, R. Meucci and F. T. Arecchi, “Synchronization of uncoupled excitable systems induced by white and coloured noise”. New Journal of Physics, 12 053040 (2010).

Samuel Zambrano, M. A. F. Sanjuán and J. A. Yorke, “Partial Control of Chaotic Systems”. Phys. Rev. E 77, 055201(R) (2008).

J. M. Amigó, Samuel Zambrano and M. A. F. Sanjuán. “True and false forbidden patterns in deterministic and random dynamics”, Europhys. Lett. 79, 50001 (2007).

Il titolare del presente curriculum vitae, pubblicato online sul portale, è garante in via esclusiva della correttezza e della veridicità dei dati e delle informazioni in esso riportate e del loro eventuale e puntuale aggiornamento. Egli è dunque il diretto ed unico responsabile dei contenuti indicati nei propri curricula.