Cremona Ottavio Professore ordinarioMedicineBIO/16

Curriculum Vitae
Education:
1993:  Ph.D. in Human Oncology, University of Torino, School of Medicine
1989:  Medical Doctor, “Summa cum laude” and Special Award, University of Torino (Italy), School of Medicine

Research appointments:
October 2010 – present: Head of the Unit: “Mouse Functional Genetics” – DIBIT2
September 2005-2010: Group Leader, IFOM, FIRC Institute of Molecular Oncology, Milano, Italy.
1995- 2005: Visiting Scientist, Department of Cell Biology and Howard Hughes Medical Institute (laboratory of Dr. Pietro De Camilli), YaleUniversity, New Haven, CT, USA
1994-2001: Assistant Professor, Department of Medical Sciences, Università del Piemonte Orientale “A. Avogadro”.
1992-1993: Maitre Assistant, Département de Pathologie, Centre Médicale Universitaire, Faculté de Médecine, Université de Genève.
1990-1991: Post-doctoral Research Fellow, Laboratory of Dr. Giulio Gabbiani, Département de Pathologie, Université de Genève, Suisse.
1989-92: Graduate student, Laboratory of Dr. Pier Carlo Marchisio, Department of Biomedical Sciences and Human Oncology, University of Torino.
1986-89: Undergraduate student, Laboratory of Dr. Pier Carlo Marchisio, Department of Biomedical Sciences and Human Oncology, University of Torino.

Teaching appointments:
October 2007 – present: Full Professor of Human Anatomy, Università Vita – Salute San Raffaele, Milano, Italy
2001- 2007: Associate Professor of Human Anatomy, Università Vita e Salute San Raffaele, Milano, Italy
1997-2004: Teaching activity at the PhD Program of Cell Biology – YaleUniversity, New Haven, CT, USA
1994-2001: Assistant Professor of Histology and Embryology, School of Medicine, Università del Piemonte Orientale “A. Avogadro”. .
1992-1993: Maitre Assistant, Pathology, Université de Genève, Suisse.

Honors and awards:
Member of the BolognaAcademy of Sciences (July 19th, 2010)

Present field of research:
Endocytosis has been traditionally considered as a mechanism to maintain plasma membrane homeostasis and internalize extracellular components. This view has been considerably expanded in the last few years when it became evident that endocytosis also acts as a key regulatory platform for fine-tuning of intracellular signaling. In this respect, endocytosis does not just terminate proliferative signals by internalizing growth factor receptors but it propagates them to intracellular compartments, it coordinates with other fundamental cellular functions including actin dynamics, it provides spatial restriction to signaling responses emanating from membrane receptors and it shapes cell-fate determination signals.
The multiplicity of endocytic actions relies on a highly organized network of molecular interactions that has been mainly investigated by biochemical and functional approaches in vitro. By these means, it has become evident that there is a large array of accessory factors of endocytosis that get recruited to the clathrin coat at specific stages of its assembly and/or remodeling, and that act as hubs for a variety of interactions with molecules involved in key cellular functions. A key question in the field is to understand the impact of these scaffolds and their interactions not only in a cellular context but also in the living animal. Our laboratory, together with the Pietro De Camilli laboratory (YaleUniversity), has pioneered a genetic approach to study the endocytic process and how it is coordinated with other fundamental cellular functions, including synaptic transmission and cell signaling. We generated and partially characterized several mouse models in the field, including the synaptojanin1, amphiphysin1, dynamin1/2/3, epsin1/2/3 and endophilin1/2/3 KO mice.

List of the 5 most representative publications.
Chen, H., G. Ko, A. Zatti, G. Di Giacomo, L. Liu, E. Raiteri, E. Perucco, C. Collesi, W. Min, C. Zeiss, P. De Camilli and O. Cremona, Embryonic arrest at midgestation and disruption of Notch signaling produced by the absence of both epsin 1 and epsin 2 in mice. Proc Natl Acad Sci U S A, 2009. 106(33): p. 13838-43.
Ferguson, S.M., A. Raimondi, S. Paradise, H. Shen, K. Mesaki, A. Ferguson, O. Destaing, G. Ko, J. Takasaki, O. Cremona, O.T. E and P. De Camilli, Coordinated actions of actin and BAR proteins upstream of dynamin at endocytic clathrin-coated pits. Dev Cell, 2009. 17(6): p. 811-22.
Ferguson, S.M., G. Brasnjo, M. Hayashi, M. Wolfel, C. Collesi, S. Giovedi, A. Raimondi, L.W. Gong, P. Ariel, S. Paradise, E. O’Toole, R. Flavell, O. Cremona, G. Miesenbock, T.A. Ryan and P. De Camilli, A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis. Science, 2007. 316(5824): p. 570-4.
Cremona, O. and P. De Camilli, Phosphoinositides in membrane traffic at the synapse. J Cell Sci, 2001. 114(Pt 6): p. 1041-52.
Cremona, O., G. Di Paolo, M.R. Wenk, A. Luthi, W.T. Kim, K. Takei, L. Daniell, Y. Nemoto, S.B. Shears, R.A. Flavell, D.A. McCormick and P. De Camilli, Essential role of phosphoinositide metabolism in synaptic vesicle recycling. Cell, 1999. 99(2): p. 179-88.

Lista delle 5 pubblicazioni più rappresentative.
Chen, H., G. Ko, A. Zatti, G. Di Giacomo, L. Liu, E. Raiteri, E. Perucco, C. Collesi, W. Min, C. Zeiss, P. De Camilli and O. Cremona, Embryonic arrest at midgestation and disruption of Notch signaling produced by the absence of both epsin 1 and epsin 2 in mice. Proc Natl Acad Sci U S A, 2009. 106(33): p. 13838-43.
Ferguson, S.M., A. Raimondi, S. Paradise, H. Shen, K. Mesaki, A. Ferguson, O. Destaing, G. Ko, J. Takasaki, O. Cremona, O.T. E and P. De Camilli, Coordinated actions of actin and BAR proteins upstream of dynamin at endocytic clathrin-coated pits. Dev Cell, 2009. 17(6): p. 811-22.
Ferguson, S.M., G. Brasnjo, M. Hayashi, M. Wolfel, C. Collesi, S. Giovedi, A. Raimondi, L.W. Gong, P. Ariel, S. Paradise, E. O’Toole, R. Flavell, O. Cremona, G. Miesenbock, T.A. Ryan and P. De Camilli, A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis. Science, 2007. 316(5824): p. 570-4.
Cremona, O. and P. De Camilli, Phosphoinositides in membrane traffic at the synapse. J Cell Sci, 2001. 114(Pt 6): p. 1041-52.
Cremona, O., G. Di Paolo, M.R. Wenk, A. Luthi, W.T. Kim, K. Takei, L. Daniell, Y. Nemoto, S.B. Shears, R.A. Flavell, D.A. McCormick and P. De Camilli, Essential role of phosphoinositide metabolism in synaptic vesicle recycling. Cell, 1999. 99(2): p. 179-88.